AmProtein Expression Technology
AmProtein has discovered a universal GC-rich method that leads to a strong DNA physical structure. This strong DNA structure makes the gene promoter and enhancer continuously exposed to transcriptional machinery and factors by preventing chromatin condensing. It is truly a 'chromatin opening element' for possibly all eukaryotic gene expression.
FEATURES OF AMPROTEIN'S PMH 3 - 5 VECTOR SERIES:
- One can reach commercial level protein titers after only a few laboratory-scale stable gene transfections.
- A known universal vector set, working in mammalian, chicken and fish cells.
- Reaching 50-300pg/cell/day in 96-well plates for larger proteins (ie. antibodies) and 20-90pg/cell/day in 96-well plates for small proteins (ie. FSH and HBsAg).
- Used successfully in over 20 recombinant proteins and antibodies.
|
Project Name |
Stable production speed (pg/cell/day) |
Molecular Size |
Posttranslational modification |
Other Features |
|
Erbitux (anti-EGFR) |
80 |
Large |
|
Antibody |
|
Fc-IL-1ra |
120 |
Medium |
|
Fc -Fusion Protein |
|
Campath (anti-CD52) |
118 |
Large |
|
Antibody |
|
EPO |
72 |
Small |
Heavily glycosylated |
|
|
EPO-HyperG |
300 |
Small |
Heavily glycosylated |
Fusion molecule |
|
Enbrel (TNFR2-Fc) |
116 |
Large |
|
|
|
HBsAg |
90 |
Small |
|
Membrane lipid vesicle |
AMPROTEIN ALSO PROVIDES THE EFFECTIVE GC-RICH FRAGMENTS FOR ENGINEERING OTHER VECTORS, SUCH AS PLANT VECTORS.
LICENSING
This vector series can be licensed out commercially for commercial research and commercial product development.
AmProtein values academic research that continues to benefit mankind and therefore provides affordable academic licenses.
AmProtein Research License - Vector Series for Mammalian, Chicken & Fish Cells
United States & Europe
- Small/Start-up Company or University Research Lab - $10K/yr - Academia will receive further discounted rates from those listed on this site.
- Medium Company - $50K/yr
- Large Company - $100K/yr
Developing Countries
- Small/Start-up Company or University Research Lab - $5K/yr - Academia will receive further discounted rates from those listed on this site.
- Medium Company - $25K/yr
- Large Company - $50K/yr
AmProtein Commercial-use License - Vector Series for Mammalian, Chicken & Fish Cell
United States & Europe
Depending on the product type, market location, and market size the commercial license will be available for a one-time payment from $1 million - $3 million.
Developing Countries
Depending on the product type, market location, and market size the commercial license will be available for a one-time payment from $0.5 million - $1.5 million.
NOTE: THESE PRICES ARE ONLY AVAILABLE TO COMPANIES AND RESEARCH INSTITUTIONS THAT HAVE NOT USED OUR TECHNOLOGY WITHOUT OUR PRIOR CONSENT.
Making History
The development of the CHO dhfr- cell lines to increase expression titers was the first revolution in mammalian gene expression to transform research into a commercial process. However, with it came a tedious and costly cell clone screening process. Our pMH vector series has surpassed current expression technologies by delivering commercial level protein titers after only several stable gene transfections. This is the SECOND REVOLUTION for possibly the entire field of eukaryotic gene expression.