AmProtein CURRENT Perfusion Bioreactors
By using the CURRENT Bioreactor as a DO generator and suspension seed culture vessel, we have developed an extremely high-density cell culture system for protein and antibody production and alternatively, carrier-attached cells for vaccine production.
Features of the Perfusion Column
The perfusion column was pre-filled with affordable, single-use polymer fiber carriers. These perfusion column bags are shipped gamma-irradiated and ready-to-use once unpacked. The perfusion columns are affiliated attachments for the 5L, 50L and 150L CURRENT Bioreactors and are thus available in sizes according to bioreactor volume.
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The most important features of the Perfusion Bioreactor include:
- Extremely high cell density in perfusion column filled with polymer fiber carriers reduces the need for serum and supports high production and high density during the high-speed perfusion.
- Harvest 1 bioreactor vessel volume (5L, 50L, 150L respectively) within 1 to 3 days, replacing a bioreactor 20 times larger in capacity. It is particularly suited for industrial applications.
- Besides its capabilities to produce large quantities of recombinant protein and antibodies, the perfusion bioreactor will replace current, outdated methods of vaccine production.
- Serum-free suspension CHO-K1 cells and attached CHO-K1 cells successfully tested for protein and antibody production.
- VERO, MDCK, Chicken, Fish cells, as well as several others have been successfully tested for animal and human vaccine production.
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Cell line name |
5L perfusion bioreactor (cell#/gram X paper-carrier total weight 150 grams) |
50L perfusion bioreactor (cell#/gram X paper-carrier total weight 1200 grams) |
150L perfusion bioreactor (cell#/gram X paper-carrier total weight 3600 grams) |
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CHO-K1 |
13.7±3.2 X 108cells/g X 150g |
16.4±4.0 X 108cells/g X 1200g |
Pending |
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CHO-S |
21.0±3.4 X 108cells/g X 150g |
25.0±4.2 X 108cells/g X 1200g |
18.0±5.0 X 108cells/g X 3600g |
Conclusion: 150L paper-carrier perfusion bioreactor equals a 1500L fed-batch bioreactor.
Advantage: Single-use paper-carrier perfusion bioreactor is characterized by a high cell density, thus having strong cell growth and survival features. It is ideal for the industrial manufacture of protein and antibody drugs.
"Current" Paper-carrier Perfusion Bioreactor Cell Culture Data
(cell density/gram paper-carrier by paper-carrier total weight) for vaccine production
| Cell line name | 5L perfusion bioreactor (cell#/gram×paper-carrier total weight 150 grams) | 50L perfusion bioreactor (cell#/gram×paper-carrier total weight 1200 grams) | 150L perfusion bioreactor (cell#/gram×paper-carrier total weight 3600 grams) |
| VERO (human) | 6.0±1.2×108cells/g×150g | 6.5±2.1×108cells/g×1200g | Pending |
| MDCK (human) | 5.0±1.0×108cells/g×150g | Pending | Pending |
| 2BS (diploid) | 3.0×108 cells/g×150g | Pending | Pending |
| Mark145 (animal) | 3.5±0.8×108cells/g×150g | Pending | Pending |
| ST1 (animal) | 4.0±1.0×108cells/g×150g | Pending | Pending |
| DF-1 (chicken) | 2.5±0.3×108cells/g×150g | Pending | Pending |
| CIK (fish) | 1.0±0.2×108cells/g×150g | Pending | Pending |
| EPC (fish) | 1.2±0.3×108cells/g×150g | Pending | Pending |
Conclusions:
1) A 50L paper-carrier perfusion bioreactor equals a production plant of 1200 large roller bottles and is perfect for larger-scale animal and human vaccine production, including chicken and fish. It is also great for rapid vaccine production of a deadly virus outbreak (such as SARS and bird flu outbreaks) after the virus DNA extracted.
2) Single-use and low cost.
Advantages:
1) Due to extremely high cell density, cell-cell growth support reaches the peak greatly for low-growth rate vaccine production cell lines;
2) Compared with roller-bottles and micro-carrier methods, seeding method (cell wash & trypsin-digest) for scale up is greatly simplified.
3) DO,pH,temperature control and cell line-specific medium optimization.
Mini Bioreactors to Support Process Development in Perfusion Bioreactors
AmProtein has developed a single-use, plastic miniature rolling bioreactor system for our customers to study the parameters involved in the process development stages for this perfusion system based on these polymer fiber paper carriers.
- Each minibioreactor vessel contains 20cm3 volume pre-filled with carriers and gamma-irradiated, ready-to-use.
- Each minibioreactor system can handle 45 vessels for efficient process development studies.
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Note: Cell-specific culture media for the above cells and the related process technology can be supplied upon request.